Starting observations with 4x magnification is not a universal requirement, but it can be beneficial in certain contexts, particularly in microscopy. Here are a few reasons why starting with 4x magnification may be important or advantageous:
Field of view: Lower magnifications provide a wider field of view, allowing you to observe a larger area. This is particularly useful when you're trying to locate a specific object or get a general overview of a sample. Starting with 4x magnification helps you establish spatial awareness and locate areas of interest before zooming in further.
Depth of field: Higher magnifications often have a shallower depth of field, meaning that only a narrow plane will be in focus at a time. By starting with 4x magnification, you can initially get a broader view of the sample, which may help you identify the most suitable region to focus on later.